In Vitro and In Vivo Comparison of Selected Ga-68 and Zr-89 Labelled Siderophores.

ABSTRACT:

Purpose

Some [(68)Ga]siderophores show promise in specific and sensitive imaging of infection. Here, we compare the in vitro and in vivo behaviour of selected Ga-68 and Zr-89 labelled siderophores.

Procedures

Radiolabelling was performed in HEPES or sodium acetate buffer systems. Radiochemical purity of labelled siderophores was determined using chromatography. Partition coefficients, in vitro stability and protein binding affinities were determined. Ex vivo biodistribution and animal imaging was studied in mice.

Results

Certain differences among studied siderophores were observed in labelling efficiency. Protein binding and stability tests showed highest stabilities and lowest protein binding affinities for Ga-68 and [(89)Zr]triacetylfusarinine C (TAFC). All studied

SUBMITTER: Petrik M 

PROVIDER: S-EPMC4870302 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature