Protein expression changes induced in a malignant melanoma cell line by the curcumin analogue compound D6.

ABSTRACT:

Background

We have previously demonstrated that the hydroxylated biphenyl compound D6 (3E,3'E)-4,4'-(5,5',6,6'-tetramethoxy-[1,1'-biphenyl]-3,3'-diyl)bis(but-3-en-2-one), a structural analogue of curcumin, exerts a strong antitumor activity on melanoma cells both in vitro and in vivo. Although the mechanism of action of D6 is yet to be clarified, this compound is thought to inhibit cancer cell growth by arresting the cell cycle in G2/M phase, and to induce apoptosis through the mitochondrial intrinsic pathway. To investigate the changes in protein expression induced by exposure of melanoma cells to D6, a differential proteomic study was carried out on D6-treated and untreated primary melanoma LB24Dagi cells.

Methods

Proteins were fractionated by SDS-PAGE and subjected to in

SUBMITTER: Pisano M 

PROVIDER: S-EPMC4870815 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature