High Level Secretion of Laccase (LccH) from a Newly Isolated White-Rot Basidiomycete, Hexagonia hirta MSF2.

ABSTRACT: Newer and novel laccases attract considerable attention due to its promising and valuable multiple applications in biotech industry. This present investigation documents, for the first time, on high level extracellular secretion of laccase (LccH) in newly isolated wood-degrading basidiomycete Hexagonia hirta MSF2. LccH was optimally active at 40°C in citrate phosphate buffer with a pH of 3.4. Optimized Cu(2+) in glucose yeast extract (GY) medium enhanced the LccH production by H. hirta to 1944.44 U.ml(-1). A further increment in LccH activity of 5671.30 U.ml(-1) was achieved by the addition of a phenolic inducer, 2,5 Xylidine. Zymogram and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of LccH revealed that LccH is a monomer with a molecular mass of 66 kDa. M

SUBMITTER: Kandasamy S 

PROVIDER: S-EPMC4870842 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature