Both Complexity and Location of DNA Damage Contribute to Cellular Senescence Induced by Ionizing Radiation.

ABSTRACT: Persistent DNA damage is considered as a main cause of cellular senescence induced by ionizing radiation. However, the molecular bases of the DNA damage and their contribution to cellular senescence are not completely clear. In this study, we found that both heavy ions and X-rays induced senescence in human uveal melanoma 92-1 cells. By measuring senescence associated-β-galactosidase and cell proliferation, we identified that heavy ions were more effective at inducing senescence than X-rays. We observed less efficient repair when DNA damage was induced by heavy ions compared with X-rays and most of the irreparable damage was complex of single strand breaks and double strand breaks, while DNA damage induced by X-rays was mostly repaired in 24 hours and the remained damage was preferentially

SUBMITTER: Zhang X 

PROVIDER: S-EPMC4871470 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature