Correlative nonlinear optical microscopy and infrared nanoscopy reveals collagen degradation in altered parchments.

ABSTRACT: This paper presents the correlative imaging of collagen denaturation by nonlinear optical microscopy (NLO) and nanoscale infrared (IR) spectroscopy to obtain morphological and chemical information at different length scales. Such multiscale correlated measurements are applied to the investigation of ancient parchments, which are mainly composed of dermal fibrillar collagen. The main issue is to characterize gelatinization, the ultimate and irreversible alteration corresponding to collagen denaturation to gelatin, which may also occur in biological tissues. Key information about collagen and gelatin signatures is obtained in parchments and assessed by characterizing the denaturation of pure collagen reference samples. A new absorbing band is observed near the amide I band in the IR spectra,

SUBMITTER: Latour G 

PROVIDER: S-EPMC4872060 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature