Reconstruction of gene regulatory networks reveals chromatin remodelers and key transcription factors in tumorigenesis.

ABSTRACT:

Background

Alterations in genetic and epigenetic landscapes are known to contribute to the development of different types of cancer. However, the mechanistic links between transcription factors and the epigenome which coordinate the deregulation of gene networks during cell transformation are largely unknown.

Methods

We used an isogenic model of stepwise tumorigenic transformation of human primary cells to monitor the progressive deregulation of gene networks upon immortalization and oncogene-induced transformation. We applied a systems biology approach by combining transcriptome and epigenome data for each step during transformation and integrated transcription factor-target gene associations in order to reconstruct the gene regulatory networks that are at the basis of the

SUBMITTER: Malysheva V 

PROVIDER: S-EPMC4872343 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature