Time-Dependent Effects of Ethanol on BK Channel Expression and Trafficking in Hippocampal Neurons.

ABSTRACT:

Background

The large conductance Ca(2+) - and voltage-activated K(+) channel (BK) is an important player in molecular and behavioral alcohol tolerance. Trafficking and surface expression of ion channels contribute to the development of addictive behaviors. We have previously reported that internalization of the BK channel is a component of molecular tolerance to ethanol (EtOH).

Methods

Using primary cultures of hippocampal neurons, we combine total internal reflection fluorescence microscopy, electrophysiology, and biochemical techniques to explore how exposure to EtOH affects the expression and subcellular localization of endogenous BK channels over time.

Results

Exposure to EtOH changed the expression of endogenous BK channels in a time-dependent manner at the perim

SUBMITTER: Palacio S 

PROVIDER: S-EPMC4873156 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature