Development and characterization of 15 microsatellite markers for Cephalotaxus fortunei (Cephalotaxaceae).

ABSTRACT: PREMISE OF THE STUDY:To survey population variation and the adaptive evolution of Cephalotaxus fortunei (Cephalotaxaceae), an endemic and endangered conifer in China, microsatellite markers were developed and characterized for this species. METHODS AND RESULTS:Based on the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, 15 microsatellite markers were developed for C. fortunei, 13 of which were polymorphic within a sample of 75 individuals representing five natural populations. The number of alleles per locus ranged from one to seven. The expected and observed heterozygosities were 0.108-0.738 and 0.000-1.000, respectively. Ten polymorphic loci were also successfully amplified in C. oliveri. CONCLUSIONS:These polymorphic loci provide a valuable tool for population genetic analysis of C. fortunei, which will contribute to its management and conservation.

SUBMITTER: Wang C 

PROVIDER: S-EPMC4873268 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature