Polysome Profiling Links Translational Control to the Radioresponse of Glioblastoma Stem-like Cells.

ABSTRACT: Changes in polysome-bound mRNA (translatome) are correlated closely with changes in the proteome in cells. Therefore, to better understand the processes mediating the response of glioblastoma to ionizing radiation (IR), we used polysome profiling to define the IR-induced translatomes of a set of human glioblastoma stem-like cell (GSC) lines. Although cell line specificity accounted for the largest proportion of genes within each translatome, there were also genes that were common to the GSC lines. In particular, analyses of the IR-induced common translatome identified components of the DNA damage response, consistent with a role for the translational control of gene expression in cellular radioresponse. Moreover, translatome analyses suggested that IR enhanced cap-dependent translation pro

SUBMITTER: Wahba A 

PROVIDER: S-EPMC4873349 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature