Elucidation of the Roles of Tumor Integrin β1 in the Extravasation Stage of the Metastasis Cascade.

ABSTRACT: Tumor integrin β1 (ITGB1) contributes to primary tumor growth and metastasis, but its specific roles in extravasation have not yet been clearly elucidated. In this study, we engineered a three-dimensional microfluidic model of the human microvasculature to recapitulate the environment wherein extravasation takes place and assess the consequences of β1 depletion in cancer cells. Combined with confocal imaging, these tools allowed us to decipher the detailed morphology of transmigrating tumor cells and associated endothelial cells in vitro at high spatio-temporal resolution not easily achieved in conventional transmigration assays. Dynamic imaging revealed that β1-depleted cells lacked the ability to sustain protrusions into the subendothelial matrix in contrast with control cells. Specifica

SUBMITTER: Chen MB 

PROVIDER: S-EPMC4873393 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature