Neurite outgrowth on electrospun PLLA fibers is enhanced by exogenous electrical stimulation.

ABSTRACT:

Objective

Both electrical stimuli (endogenous and exogenous) and topographical cues are instructive to axonal extension. This report, for the first time, investigated the relative dominance of directional topographical guidance cues and directional electrical cues to enhance and/or direct primary neurite extension. We hypothesized the combination of electrical stimulation with electrospun fiber topography would induce longer neurite extension from dorsal root ganglia neurons than the presence of electrical stimulation or aligned topography alone.

Approach

To test the hypothesis, neurite outgrowth was examined on laminin-coated poly-L-lactide films or electrospun fibers (2 µm in diameter) in the presence or absence of electrical stimulation. Immunostained neurons were semi-au

SUBMITTER: Koppes AN 

PROVIDER: S-EPMC4873603 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature