LAMP Detection Assays for Boxwood Blight Pathogens: A Comparative Genomics Approach.

ABSTRACT: Rapid and accurate molecular diagnostic tools are critical to efforts to minimize the impact and spread of emergent pathogens. The identification of diagnostic markers for novel pathogens presents several challenges, especially in the absence of information about population diversity and where genetic resources are limited. The objective of this study was to use comparative genomics datasets to find unique target regions suitable for the diagnosis of two fungal species causing a newly emergent blight disease of boxwood. Candidate marker regions for loop-mediated isothermal amplification (LAMP) assays were identified from draft genomes of Calonectria henricotiae and C. pseudonaviculata, as well as three related species not associated with this disease. To increase the probability of identif

SUBMITTER: Malapi-Wight M 

PROVIDER: S-EPMC4873745 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature