A protein expression system for tandem affinity purification in Xanthomonas citri subsp. citri.

ABSTRACT: Citrus canker, caused by the Gram-negative bacterium Xanthomonas citri subsp. citri (Xac), is one of the most devastating diseases to affect citrus crops. There is no treatment for citrus canker; effective control against the spread of Xac is usually achieved by the elimination of affected plants along with that of asymptomatic neighbors. An in depth understanding of the pathogen is the keystone for understanding of the disease; to this effect we are committed to the development of strategies to ease the study of Xac. Genome sequencing and annotation of Xac revealed that ∼37% of the genome is composed of hypothetical ORFs. To start a systematic characterization of novel factors encoded by Xac, we constructed integrative-vectors for protein expression specific to this bacterium. The vectors

SUBMITTER: Dantas GC 

PROVIDER: S-EPMC4874617 | biostudies-literature | 2016 Apr-Jun

REPOSITORIES: biostudies-literature