Glycan analysis by reversible reaction to hydrazide beads and mass spectrometry.

ABSTRACT: Investigation into glycoproteins and their associated glycans is the key to understanding the function of glycoproteins in biological pathways and disease development. Current methods for glycan analysis are generally based on multiple preparation processes to separate glycans from proteins and other molecules prior to analysis. During the multistep purification processes, glycans are continuously lost and the procedure increases the difficulty for accurate quantitative analysis of glycans. Here we describe the development of a novel technique, which uses hydrazide beads to capture glycans. It is based on the conjugation of glycans to hydrazide beads through the formation of reversible hydrazone, washing out unbound nonglycans, then releasing captured glycans by acids. The results showed t

SUBMITTER: Yang SJ 

PROVIDER: S-EPMC4876039 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature