Magnetic Optical Microarray Imager for Diagnosing Type of Diabetes in Clinical Blood Serum Samples.

ABSTRACT: Due to rapidly rising rates of diabetes and prediabetic conditions worldwide and the associated lethal complications, it is imperative to devise new diagnostic tools that reliably and directly measure insulin levels in clinical samples. Herein, we report a simple and sensitive direct imaging of insulin levels in diabetic patient samples using a surface plasmon resonance microarray imager (SPRi). To enhance sensitivity, we utilized magnetic nanoparticles (MNPs) to capture insulin from serum samples either directly or via a capture antibody immobilized on MNPs. The insulin-captured nanoparticles were allowed to bind surface insulin-antibody for detection from pixel intensity increase using a charge coupled device (CCD) built-in with the SPRi. We have compared the analytical figures-of-merit

SUBMITTER: Singh V 

PROVIDER: S-EPMC4877040 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature