Cell-Envelope Remodeling as a Determinant of Phenotypic Antibacterial Tolerance in <i>Mycobacterium tuberculosis</i>.

ABSTRACT: The mechanisms that lead to phenotypic antibacterial tolerance in bacteria remain poorly understood. We investigate whether changes in NaCl concentration toward physiologically higher values affect antibacterial efficacy against Mycobacterium tuberculosis (Mtb), the causal agent of human tuberculosis. Indeed, multiclass phenotypic antibacterial tolerance is observed during Mtb growth in physiologic saline. This includes changes in sensitivity to ethionamide, ethambutol, d-cycloserine, several aminoglycosides, and quinolones. By employing organism-wide metabolomic and lipidomic approaches combined with phenotypic tests, we identified a time-dependent biphasic adaptive response after exposure of Mtb to physiological levels of NaCl. A first rapid, extensive, and reversible phase was as

SUBMITTER: Larrouy-Maumus G 

PROVIDER: S-EPMC4877114 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature