Assembly and operation of the autopatcher for automated intracellular neural recording in vivo.

ABSTRACT: Whole-cell patch clamping in vivo is an important neuroscience technique that uniquely provides access to both suprathreshold spiking and subthreshold synaptic events of single neurons in the brain. This article describes how to set up and use the autopatcher, which is a robot for automatically obtaining high-yield and high-quality whole-cell patch clamp recordings in vivo. By following this protocol, a functional experimental rig for automated whole-cell patch clamping can be set up in 1 week. High-quality surgical preparation of mice takes ∼1 h, and each autopatching experiment can be carried out over periods lasting several hours. Autopatching should enable in vivo intracellular investigations to be accessible by a substantial number of neuroscience laboratories, and it enables labs tha

SUBMITTER: Kodandaramaiah SB 

PROVIDER: S-EPMC4877510 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature