A transcriptome-based global map of signaling pathways in the ovarian cancer microenvironment associated with clinical outcome.

ABSTRACT:

Background

Soluble protein and lipid mediators play essential roles in the tumor environment, but their cellular origins, targets, and clinical relevance are only partially known. We have addressed this question for the most abundant cell types in human ovarian carcinoma ascites, namely tumor cells and tumor-associated macrophages.

Results

Transcriptome-derived datasets were adjusted for errors caused by contaminating cell types by an algorithm using expression data derived from pure cell types as references. These data were utilized to construct a network of autocrine and paracrine signaling pathways comprising 358 common and 58 patient-specific signaling mediators and their receptors. RNA sequencing based predictions were confirmed for several proteins and lipid mediators.

SUBMITTER: Reinartz S 

PROVIDER: S-EPMC4877997 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature