Molecular Dynamics Simulation of DNA Capture and Transport in Heated Nanopores.

ABSTRACT: The integration of local heat sources with solid-state nanopores offers new means for controlling the transmembrane transport of charged biomacromolecules. In the case of electrophoretic transport of DNA, recent experimental studies revealed unexpected temperature dependences of the DNA capture rate, the DNA translocation velocity, and the ionic current blockades produced by the presence of DNA in the nanopore. Here, we report the results of all-atom molecular dynamics simulations that elucidated the effect of temperature on the key microscopic processes governing electric field-driven transport of DNA through nanopores. Mimicking the experimental setup, we simulated the capture and subsequent translocation of short DNA duplexes through a locally heated nanopore at several temperatures and

SUBMITTER: Belkin M 

PROVIDER: S-EPMC4880514 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature