Profiling of circulating microRNAs in patients with Barrett's esophagus and esophageal adenocarcinoma.

ABSTRACT:

Background

Circulating microRNAs (miRNAs) have been suggested as novel markers for various diseases. The goal of this pilot study was to identify circulating miRNAs differentially expressed comparing Barrett's esophagus (BE), esophageal adenocarcinoma (EAC), and controls.

Methods

MicroRNA expression profiling was performed by qPCR array using plasma from six controls and eight BE and eight EAC patients. Validation was performed by analyzing the expression of six selected miRNAs, by qRT-PCR in 115 plasma samples of controls, BE, and EAC patients. Diagnostic accuracy was evaluated by area under the curve (AUC) analysis.

Results

We identified three miRNAs that were elevated in EAC and four miRNAs that were elevated in BE. Further validation showed that miRNA-382-5p was s

SUBMITTER: Bus P 

PROVIDER: S-EPMC4880635 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature