Platinum-Based Drugs and DNA Interactions Studied by Single-Molecule and Bulk Measurements.

ABSTRACT: Platinum-containing molecules are widely used as anticancer drugs. These molecules exert cytotoxic effects by binding to DNA through various mechanisms. The binding between DNA and platinum-based drugs hinders the opening of DNA, and therefore, DNA duplication and transcription are severely hampered. Overall, impeding the above-mentioned important DNA mechanisms results in irreversible DNA damage and the induction of apoptosis. Several molecules, including multinuclear platinum compounds, belong to the family of platinum drugs, and there is a body of research devoted to developing more efficient and less toxic versions of these compounds. In this study, we combined different biophysical methods, including single-molecule assays (magnetic tweezers) and bulk experiments (ultraviolet absorpti

SUBMITTER: Salerno D 

PROVIDER: S-EPMC4880801 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature