Effects of glucose on lactose synthesis in mammary epithelial cells from dairy cow.

ABSTRACT:

Background

Lactose, as the primary osmotic component in milk, is the major determinant of milk volume. Glucose is the primary precursor of lactose. However, the effect of glucose on lactose synthesis in dairy cow mammary glands and the mechanism governing this process are poorly understood.

Results

Here we showed that glucose has the ability to induce lactose synthesis in dairy cow mammary epithelial cells, as well as increase cell viability and proliferation. A concentration of 12 mM glucose was the optimum concentration to induce cell growth and lactose synthesis in cultured dairy cow mammary epithelial cells. In vitro, 12 mM glucose enhanced lactose content, along with the expression of genes involved in glucose transportation and the lactose biosynthesis pathway, includi

SUBMITTER: Lin Y 

PROVIDER: S-EPMC4880877 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature