Genome-wide identification of microRNAs in pomegranate (Punica granatum L.) by high-throughput sequencing.

ABSTRACT:

Background

MicroRNAs (miRNAs), a class of small non-coding endogenous RNAs that regulate gene expression post-transcriptionally, play multiple key roles in plant growth and development and in biotic and abiotic stress response. Knowledge and roles of miRNAs in pomegranate fruit development have not been explored.

Results

Pomegranate, which accumulates a large amount of anthocyanins in skin and arils, is valuable to human health, mainly because of its antioxidant properties. In this study, we developed a small RNA library from pooled RNA samples from young seedlings to mature fruits and identified both conserved and pomegranate-specific miRNA from 29,948,480 high-quality reads. For the pool of 15- to 30-nt small RNAs, ~50 % were 24 nt. The miR157 family was the most abundant,

SUBMITTER: Saminathan T 

PROVIDER: S-EPMC4880961 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature