Proteomic Study of Entamoeba histolytica Trophozoites, Cysts, and Cyst-Like Structures.

ABSTRACT: The cyst stage of Entamoeba histolytica is a promising therapeutic target against human amoebiasis. Our research team previously reported the production in vitro of Cyst-Like Structures (CLS) sharing structural features with cysts, including rounded shape, size reduction, multinucleation, and the formation of a chitin wall coupled to the overexpression of glucosamine 6-phosphate isomerase, the rate-limiting enzyme of the chitin synthesis pathway. A proteomic study of E. histolytica trophozoites, cysts, and in vitro-produced CLS is reported herein to determine the nature of CLS, widen our knowledge on the cyst stage, and identify possible proteins and pathways involved in the encystment process. Total protein extracts were obtained from E. histolytica trophozoites, CLS, and partially purifi

SUBMITTER: Luna-Nacar M 

PROVIDER: S-EPMC4882050 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature