Characterizing Strain Variation in Engineered E. coli Using a Multi-Omics-Based Workflow.

ABSTRACT: Understanding the complex interactions that occur between heterologous and native biochemical pathways represents a major challenge in metabolic engineering and synthetic biology. We present a workflow that integrates metabolomics, proteomics, and genome-scale models of Escherichia coli metabolism to study the effects of introducing a heterologous pathway into a microbial host. This workflow incorporates complementary approaches from computational systems biology, metabolic engineering, and synthetic biology; provides molecular insight into how the host organism microenvironment changes due to pathway engineering; and demonstrates how biological mechanisms underlying strain variation can be exploited as an engineering strategy to increase product yield. As a proof of concept, we present th

SUBMITTER: Brunk E 

PROVIDER: S-EPMC4882250 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature