Ontology highlight
ABSTRACT:
SUBMITTER: Suzuki M
PROVIDER: S-EPMC4882641 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Suzuki Mami M Yamanoi Ayaka A Machino Yusuke Y Ootsubo Michiko M Izawa Ken-ichi K Kohroki Junya J Masuho Yasuhiko Y
Journal of biochemistry 20150808 1
The Fc domain of human IgG1 binds to Fcγ receptors (FcγRs) to induce effector functions such as phagocytosis. There are four interchain disulfide bonds between the H and L chains. In this study, the disulfide bonds within the IgG1 trastuzumab (TRA), which is specific for HER2, were cleaved by mild S-sulfonation or by mild reduction followed by S-alkylation with three different reagents. The cleavage did not change the binding activities of TRA to HER2-bearing SK-BR-3 cells. The binding activitie ...[more]