STIM1 activation is regulated by a 14 amino acid sequence adjacent to the CRAC activation domain.

ABSTRACT: Oligomerization of the Ca²⁺ sensor, STIM1, in the endoplasmic reticulum (ER) membrane, caused by depletion of ER Ca²⁺ stores, results in STIM1 coupling to the plasma membrane Ca²⁺ channel protein, Orai1, to activate Ca²⁺ influx in a process known as store-operated Ca²⁺ entry. We use fluorimetry-based fluorescence resonance energy transfer (FRET) to monitor changes in STIM1 oligomerization in COS7 cells transfected with STIM1 constructs containing selected truncations, deletions, and point mutations, and labeled with donor and acceptor fluorescent proteins at either the luminal (N-terminal) or the cytoplasmic (C-terminal) ends. Our results with sequential truncations of STIM1 from the C-terminus support previous evidence that the CRAC a

SUBMITTER: Korzeniowski MK 

PROVIDER: S-EPMC4883682 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature