An in planta, Agrobacterium-mediated transient gene expression method for inducing gene silencing in rice (Oryza sativa L.) leaves.

ABSTRACT:

Background

Localized introduction and transient expression of T-DNA constructs mediated by agro-infiltration of leaf tissues has been largely used in dicot plants for analyzing the transitivity and the cell-to cell movement of the RNAi signal. In cereals, however, the morphology of the leaf and particularly the structure of the leaf epidermis, prevent infiltration of a bacterial suspension in cells by simple pressure, a method otherwise successful in dicots leaves. This study aimed at establishing a rapid method for the functional analysis of rice genes based on the triggering of RNA interference (RNAi) following Agrobacterium-mediated transient transformation of leaves.

Results

Using an agro-infection protocol combining a wound treatment and a surfactant, we were able to ob

SUBMITTER: Andrieu A 

PROVIDER: S-EPMC4883685 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature