RNAi mediated down regulation of myo-inositol-3-phosphate synthase to generate low phytate rice.

ABSTRACT:

Background

Phytic acid (InsP6) is considered as the major source of phosphorus and inositol phosphates in cereal grains. Reduction of phytic acid level in cereal grains is desirable in view of its antinutrient properties to maximize mineral bioavailability and minimize the load of phosphorus waste management. We report here RNAi mediated seed-specific silencing of myo-inositol-3-phosphate synthase (MIPS) gene catalyzing the first step of phytic acid biosynthesis in rice. Moreover, we also studied the possible implications of MIPS silencing on myo-inositol and related metabolism, since, first step of phytic acid biosynthesis is also the rate limiting step of myo-inositol synthesis, catalyzed by MIPS.

Results

The resulting transgenic rice plants (T3) showed a 4.59 fold down re

SUBMITTER: Ali N 

PROVIDER: S-EPMC4883737 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature