Engineering Escherichia coli for production of 4-hydroxymandelic acid using glucose-xylose mixture.

ABSTRACT:

Background

4-Hydroxymandelic acid (4-HMA) is a valuable aromatic fine chemical and widely used for production of pharmaceuticals and food additives. 4-HMA is conventionally synthesized by chemical condensation of glyoxylic acid with excessive phenol, and the process is environmentally unfriendly. Microbial cell factory would be an attractive approach for 4-HMA production from renewable and sustainable resources.

Results

In this study, a biosynthetic pathway for 4-HMA production was constructed by heterologously expressing the fully synthetic 4-hydroxymandelic acid synthase (shmaS) in our L-tyrosine-overproducing Escherichia coli BKT5. The expression level of shmaS was optimized to improve 4-HMA production by fine tuning of four promoters of different strength combined with t

SUBMITTER: Li FF 

PROVIDER: S-EPMC4884394 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature