Potential applications of next generation DNA sequencing of 16S rRNA gene amplicons in microbial water quality monitoring.

ABSTRACT: The applicability of next generation DNA sequencing (NGS) methods for water quality assessment has so far not been broadly investigated. This study set out to evaluate the potential of an NGS-based approach in a complex catchment with importance for drinking water abstraction. In this multi-compartment investigation, total bacterial communities in water, faeces, soil, and sediment samples were investigated by 454 pyrosequencing of bacterial 16S rRNA gene amplicons to assess the capabilities of this NGS method for (i) the development and evaluation of environmental molecular diagnostics, (ii) direct screening of the bulk bacterial communities, and (iii) the detection of faecal pollution in water. Results indicate that NGS methods can highlight potential target populations for diagnostics an

SUBMITTER: Vierheilig J 

PROVIDER: S-EPMC4884447 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature