Enzymatic passaging of human embryonic stem cells alters central carbon metabolism and glycan abundance.

ABSTRACT: To realize the potential of human embryonic stem cells (hESCs) in regenerative medicine and drug discovery applications, large numbers of cells that accurately recapitulate cell and tissue function must be robustly produced. Previous studies have suggested that genetic instability and epigenetic changes occur as a consequence of enzymatic passaging. However, the potential impacts of such passaging methods on the metabolism of hESCs have not been described. Using stable isotope tracing and mass spectrometry-based metabolomics, we have explored how different passaging reagents impact hESC metabolism. Enzymatic passaging caused significant decreases in glucose utilization throughout central carbon metabolism along with attenuated de novo lipogenesis. In addition, we developed and validated a

SUBMITTER: Badur MG 

PROVIDER: S-EPMC4885641 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature