A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves.
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ABSTRACT:
SUBMITTER: Rodriguez J
PROVIDER: S-EPMC4885664 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Publications
A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves.
Rodríguez Jéssica J Mosquera Jesús J García-Fandiño Rebeca R Vázquez M Eugenio ME Mascareñas José L JL
Chemical science 20160205 5
We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and selectivity to a long composite sequence of 13 base pairs (TCAT-AATT-GAGAG). ...[more]
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