Identification of Quantitative Proteomic Differences between Mycobacterium tuberculosis Lineages with Altered Virulence.

ABSTRACT: Evidence currently suggests that as a species Mycobacterium tuberculosis exhibits very little genomic sequence diversity. Despite limited genetic variability, members of the M. tuberculosis complex (MTBC) have been shown to exhibit vast discrepancies in phenotypic presentation in terms of virulence, elicited immune response and transmissibility. Here, we used qualitative and quantitative mass spectrometry tools to investigate the proteomes of seven clinically-relevant mycobacterial strains-four M. tuberculosis strains, M. bovis, M. bovis BCG, and M. avium-that show varying degrees of pathogenicity and virulence, in an effort to rationalize the observed phenotypic differences. Following protein preparation, liquid chromatography mass spectrometry (LC MS/MS) and data capture were carried out

SUBMITTER: Peters JS 

PROVIDER: S-EPMC4885829 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature