A novel rodent model of posterior ischemic optic neuropathy.

ABSTRACT:

Objectives

To develop a reliable, reproducible rat model of posterior ischemic optic neuropathy (PION) and study the cellular responses in the optic nerve and retina.

Methods

Posterior ischemic optic neuropathy was induced in adult rats by photochemically induced ischemia. Retinal and optic nerve vasculature was examined by fluorescein isothiocyanate–dextran extravasation. Tissue sectioning and immunohistochemistry were used to investigate the pathologic changes. Retinal ganglion cell survival at different times after PION induction, with or without neurotrophic application, was quantified by fluorogold retrograde labeling.

Results

Optic nerve injury was confirmed after PION induction, including local vascular leakage, optic nerve edema, and cavernous degeneration. Im

SUBMITTER: Wang Y 

PROVIDER: S-EPMC4885914 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature