The Statistical Value of Raw Fluorescence Signal in Luminex xMAP Based Multiplex Immunoassays.

ABSTRACT: Tissue samples (plasma, saliva, serum or urine) from 169 patients classified as either normal or having one of seven possible diseases are analysed across three 96-well plates for the presences of 37 analytes using cytokine inflammation multiplexed immunoassay panels. Censoring for concentration data caused problems for analysis of the low abundant analytes. Using fluorescence analysis over concentration based analysis allowed analysis of these low abundant analytes. Mixed-effects analysis on the resulting fluorescence and concentration responses reveals a combination of censoring and mapping the fluorescence responses to concentration values, through a 5PL curve, changed observed analyte concentrations. Simulation verifies this, by showing a dependence on the mean florescence response and

SUBMITTER: Breen EJ 

PROVIDER: S-EPMC4886638 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature