Quantifying radiation damage in biomolecular small-angle X-ray scattering.

ABSTRACT: Small-angle X-ray scattering (SAXS) is an increasingly popular technique that provides low-resolution structural information about biological macromolecules in solution. Many of the practical limitations of the technique, such as minimum required sample volume, and of experimental design, such as sample flow cells, are necessary because the biological samples are sensitive to damage from the X-rays. Radiation damage typically manifests as aggregation of the sample, which makes the collected data unreliable. However, there has been little systematic investigation of the most effective methods to reduce damage rates, and results from previous damage studies are not easily compared with results from other beamlines. Here a methodology is provided for quantifying radiation damage in SAXS to pr

SUBMITTER: Hopkins JB 

PROVIDER: S-EPMC4886981 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature