Modeling Effects of RNA on Capsid Assembly Pathways via Coarse-Grained Stochastic Simulation.

ABSTRACT: The environment of a living cell is vastly different from that of an in vitro reaction system, an issue that presents great challenges to the use of in vitro models, or computer simulations based on them, for understanding biochemistry in vivo. Virus capsids make an excellent model system for such questions because they typically have few distinct components, making them amenable to in vitro and modeling studies, yet their assembly can involve complex networks of possible reactions that cannot be resolved in detail by any current experimental technology. We previously fit kinetic simulation parameters to bulk in vitro assembly data to yield a close match between simulated and real data, and then used the simulations to study features of assembly that cannot be monitored experimentally. The

SUBMITTER: Smith GR 

PROVIDER: S-EPMC4887116 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature