Disruption of a hydrogen bond network in human versus spider monkey cytochrome c affects heme crevice stability.

ABSTRACT: The hypothesis that the recent rapid evolution of primate cytochromes c, which primarily involves residues in the least stable Ω-loop (Ω-loop C, residues 40-57), stabilizes the heme crevice of cytochrome c relative to other mammals, is tested. To accomplish this goal, we have compared the properties of human and spider monkey cytochrome c and a set of four variants produced in the process of converting human cytochrome c into spider monkey cytochrome c. The global stability of all variants has been measured by guanidine hydrochloride denaturation. The stability of the heme crevice has been assessed with the alkaline conformational transition. Structural insight into the effects of the five amino acid substitutions needed to convert human cytochrome c into spider monkey cytochrome c is prov

SUBMITTER: Goldes ME 

PROVIDER: S-EPMC4887337 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature