Metabolic fingerprints of human primary endothelial and fibroblast cells.

ABSTRACT:

Introduction

Human primary cells originating from different locations within the body could differ greatly in their metabolic phenotypes, influencing both how they act during physiological/pathological processes and how susceptible/resistant they are to a variety of disease risk factors. A novel way to monitor cellular metabolism is through cell energetics assays, so we explored this approach with human primary cell types, as models of sclerotic disorders.

Objectives

In order to better understand pathophysiological processes at the cellular level, our goals were to measure metabolic pathway activities of endothelial cells and fibroblasts, and determine their metabolic phenotype profiles.

Methods

Biolog Phenotype MicroArray™ technology was used for the first time to ch

SUBMITTER: Zigon P 

PROVIDER: S-EPMC4887525 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature