A neuron-in-capillary platform for facile collection and mass spectrometric characterization of a secreted neuropeptide.

ABSTRACT: The integration of microfluidic devices-which efficiently handle small liquid volumes-with separations/mass spectrometry (MS) is an effective approach for profiling the neurochemistry occurring in selected neurons. Interfacing the microfluidic cell culture to the mass spectrometer is challenging because of geometric and scaling issues. Here we demonstrate the hyphenation of a neuron-in-capillary platform to a solid phase extraction device and off-line MS. A primary neuronal culture of Aplysia californica neurons was established directly inside a cylindrical polyimide capillary. The approach also uses a particle-embedded monolith to condition neuropeptide releasates collected from several Aplysia neurons cultured in the capillary, with the subsequent characterization of released peptides vi

SUBMITTER: Lee CY 

PROVIDER: S-EPMC4887886 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature