MicroRNA-155 contributes to shear-resistant leukocyte adhesion to human brain endothelium in vitro.

ABSTRACT:

Background

Increased leukocyte adhesion to brain endothelial cells forming the blood-brain barrier (BBB) precedes extravasation into the central nervous system (CNS) in neuroinflammatory diseases such as multiple sclerosis (MS). Previously, we reported that microRNA-155 (miR-155) is up-regulated in MS and by inflammatory cytokines in human brain endothelium, with consequent modulation of endothelial paracellular permeability. Here, we investigated the role of endothelial miR-155 in leukocyte adhesion to the human cerebral microvascular endothelial cell line, hCMEC/D3, under shear forces mimicking blood flow in vivo.

Results

Using a gain- and loss-of-function approach, we show that miR-155 up-regulation increases leukocyte firm adhesion of both monocyte and T cells to hCMEC/D

SUBMITTER: Cerutti C 

PROVIDER: S-EPMC4888311 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature