Single-cell sequencing reveals karyotype heterogeneity in murine and human malignancies.

ABSTRACT:

Background

Chromosome instability leads to aneuploidy, a state in which cells have abnormal numbers of chromosomes, and is found in two out of three cancers. In a chromosomal instable p53 deficient mouse model with accelerated lymphomagenesis, we previously observed whole chromosome copy number changes affecting all lymphoma cells. This suggests that chromosome instability is somehow suppressed in the aneuploid lymphomas or that selection for frequently lost/gained chromosomes out-competes the CIN-imposed mis-segregation.

Results

To distinguish between these explanations and to examine karyotype dynamics in chromosome instable lymphoma, we use a newly developed single-cell whole genome sequencing (scWGS) platform that provides a complete and unbiased overview of copy number

SUBMITTER: Bakker B 

PROVIDER: S-EPMC4888588 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature