Longitudinal, label-free, quantitative tracking of cell death and viability in a 3D tumor model with OCT.

ABSTRACT: Three-dimensional in vitro tumor models are highly useful tools for studying tumor growth and treatment response of malignancies such as ovarian cancer. Existing viability and treatment assessment assays, however, face shortcomings when applied to these large, complex, and heterogeneous culture systems. Optical coherence tomography (OCT) is a noninvasive, label-free, optical imaging technique that can visualize live cells and tissues over time with subcellular resolution and millimeters of optical penetration depth. Here, we show that OCT is capable of carrying out high-content, longitudinal assays of 3D culture treatment response. We demonstrate the usage and capability of OCT for the dynamic monitoring of individual and combination therapeutic regimens in vitro, including both chemothera

SUBMITTER: Jung Y 

PROVIDER: S-EPMC4888651 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature