Three-dimensional characterization of fibroblast foci in idiopathic pulmonary fibrosis.

ABSTRACT: In idiopathic pulmonary fibrosis (IPF), the fibroblast focus is a key histological feature representing active fibroproliferation. On standard 2D pathologic examination, fibroblast foci are considered small, distinct lesions, although they have been proposed to form a highly interconnected reticulum as the leading edge of a "wave" of fibrosis. Here, we characterized fibroblast focus morphology and interrelationships in 3D using an integrated micro-CT and histological methodology. In 3D, fibroblast foci were morphologically complex structures, with large variations in shape and volume (range, 1.3 × 10⁴ to 9.9 × 10⁷ μm³). Within each tissue sample numerous multiform foci were present, ranging from a minimum of 0.9 per mm³ of lung tissue to a maximu

SUBMITTER: Jones MG 

PROVIDER: S-EPMC4889020 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature