Usefulness of ELISA Methods for Assessing LPS Interactions with Proteins and Peptides.

ABSTRACT: Lipopolysaccharide (LPS), the major constituent of the outer membrane of Gram-negative bacteria, can trigger severe inflammatory responses during bacterial infections, possibly leading to septic shock. One approach to combatting endotoxic shock is to neutralize the most conserved part and major mediator of LPS activity (lipid A) with LPS-binding proteins or peptides. Although several available assays evaluate the biological activity of these molecules on LPS (e.g. inhibition of LPS-induced TNF-α production in macrophages), the development of simple and cost-effective methods that would enable preliminary screening of large numbers of potential candidate molecules is of great interest. Moreover, it would be also desirable that such methods could provide information about the possible biolog

SUBMITTER: Martinez-Sernandez V 

PROVIDER: S-EPMC4889133 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature