Genomic Signatures of Experimental Adaptation to Antimicrobial Peptides in Staphylococcus aureus.

ABSTRACT: The evolution of resistance against antimicrobial peptides has long been considered unlikely due to their mechanism of action, yet experimental selection with antimicrobial peptides (AMPs) results in rapid evolution of resistance in several species of bacteria. Although numerous studies have utilized mutant screens to identify loci that determine AMP susceptibility, there is a dearth of data concerning the genomic changes that accompany experimental evolution of AMP resistance. Using genome resequencing, we analyzed the mutations that arose during experimental evolution of resistance to the cationic AMPs iseganan, melittin, and pexiganan, as well as to a combination of melittin and pexiganan, or to the aminoglycoside antibiotic streptomycin. Analysis of 17 independently replicated Staphylo

SUBMITTER: Johnston PR 

PROVIDER: S-EPMC4889650 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature