Three-dimensional disorganization of the cancer genome occurs coincident with long-range genetic and epigenetic alterations.

ABSTRACT: A three-dimensional chromatin state underpins the structural and functional basis of the genome by bringing regulatory elements and genes into close spatial proximity to ensure proper, cell-type-specific gene expression profiles. Here, we performed Hi-C chromosome conformation capture sequencing to investigate how three-dimensional chromatin organization is disrupted in the context of copy-number variation, long-range epigenetic remodeling, and atypical gene expression programs in prostate cancer. We find that cancer cells retain the ability to segment their genomes into megabase-sized topologically associated domains (TADs); however, these domains are generally smaller due to establishment of additional domain boundaries. Interestingly, a large proportion of the new cancer-specific domain

SUBMITTER: Taberlay PC 

PROVIDER: S-EPMC4889976 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature