Dissecting the role of transforming growth factor-β1 in topmouth culter immunobiological activity: a fundamental functional analysis.

ABSTRACT: Transforming growth factor-β1 (TGF-β1) has been proven to function primarily in mammalian immunobiological activity, but information regarding the immune role of TGF-β1 in teleosts is limited. In the present study, we describe the cDNA cloning and characterization of the TGF-β1 molecule in the topmouth culter. TGF-β1 is highly expressed in immune-related tissues of the culter, including the thymus, head kidney, and spleen. The recombinant culter TGF-β1 (cTGF-β1) was successfully expressed and purified in vitro, and the effects of cTGF-β1 on the mRNA expression of pro-inflammatory cytokines, such as TNF-α and IL-1β, in the absence or presence of LPS was determined in culter peripheral blood leukocytes. cTGF-β1 was found to have bipolar properties in inflammatory reactions. Additionally, to

SUBMITTER: Qi P 

PROVIDER: S-EPMC4890032 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature