Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum.

ABSTRACT:

Background

Biofuel production from plant cell walls offers the potential for sustainable and economically attractive alternatives to petroleum-based products. Fuels from cellulosic biomass are particularly promising, but would benefit from lower processing costs. Clostridium thermocellum can rapidly solubilize and ferment cellulosic biomass, making it a promising candidate microorganism for consolidated bioprocessing for biofuel production, but increases in product yield and titer are still needed.

Results

Here, we started with an engineered C. thermocellum strain where the central metabolic pathways to products other than ethanol had been deleted. After two stages of adaptive evolution, an evolved strain was selected with improved yield and titer. On chemically defined medi

SUBMITTER: Tian L 

PROVIDER: S-EPMC4890492 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature